Keywords
Acupuncture
Equine chorionic gonadotropin
Prostaglandin F2alfa.
Equine chorionic gonadotropin
Prostaglandin F2alfa.
How to Cite
Souza, D. O., Biscarde, C. E. A., Machado, W. M., Vieira, R. L. A., Mendes, C. S., Araújo, M. L. de, … Barbosa, L. P. (2021). Application of hormonal subdoses at the Bai Hui acupoint for estrus synchronization in sheep. Semina: Ciências Agrárias, 42(4), 2359–2370. https://doi.org/10.5433/1679-0359.2021v42n4p2359
Abstract
Here, the efficiency of applying subdoses of equine chorionic gonadotropin (eCG) and prostaglandin F2 alfa (PGF2alfa) at the Bai Hui (BH) acupoint for estrus synchronization in sheep was evaluated. Thirty Santa Inês ewes received intravaginal sponges containing 60 mg medroxyprogesterone acetate for 7 days. The animals were divided into five treatments (T) (n=6): T1 (control), 132.5 ug PGF2 alfa (100% of dose) and 300 IU eCG (100% of dose), both intramuscularly (IM); T2, 39.75 ug PGF2 alfa (30% of dose) at the BH acupoint and 300 IU eCG (100% of dose) IM; T3, 132.5 ug PGF2 alfa (100% of dose) IM and 90 IU eCG (30% of dose) at the BH acupoint; T4, 39.75 ug PGF2 alfa (30% of dose) and 90 IU eCG (30% of dose), both at the BH acupoint; and T5, 39.75 ug PGF2 alfa (30% of dose) and 90 IU eCG (30% of dose) both at a false acupoint (IM, at the same location as in T1). After 12 h of sponge removal and hormone application, the ewes were subjected to monitoring for estrus and mating and ultrasound assessments of follicular growth and ovulation time every 12 h. The data were evaluated for normality and subjected to analysis of variance, considering a 5% probability. There were no differences in the percentage of animals in estrus (93.3±9.1%), interval from sponge removal to estrus onset (48.1±6.0 h), interval from sponge removal to estrus end (69.6±5.4 h), duration of estrus (24.0±4.7 h), interval from sponge removal to ovulation (73.7±5.9 h), interval from estrus onset to ovulation (26.5±2.3 h), size of the largest follicle (6.7±0.4 mm), follicular growth rate (0.8±0.1 mm•day-1), number of ovulations (1.3±0.1), and plasma progesterone levels 7 days after ovulation (9.6±2.0 ng•mL-1). The cost of the synchronization protocol per animal was US$ 4.37, 4.12, 2.96, 2.71, and 2.71 for T1, T2, T3, T4, and T5, respectively, with 37.99% cost reduction using PGF2 alfa and eCG at 30% of the conventional doses. Therefore, the application of reduced PGF2 alfa (39.75 ug) and eCG (90 IU) doses at the BH or false acupoint effectively synchronized estrus and reduced protocol cost in sheep, indicating that the conventional doses are overestimated.References
Araújo, M. L, Mendes, C. S., Biscarde, C. E. A., Biscarde, F. H. A., Rocha, L. F., Costa, L. N. F.,... Barbosa, L. P. (2019). Application of low dose of equine chorionic gonadotropin at acupoint Hou Hai for fixed-time artificial insemination in beef cows. Semina: Ciências Agrárias, 40(6), 2625-2636. doi: 10.5433/ 1679-0359.2019v40n6p2625
Araújo, R. C. S. A., Barbosa, L. P., Souza, R. S., Santana, A. L. A., Carneiro, I. M. B., Machado, W. M.,... Jesus, R. D. L. (2019). Farmacopuntura utilizando os acupontos Hou Hai e Bai Hui em protocolos de sincronização de estro em cabras. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 71(2), 529-537. doi: 10.1590/1678-4162-10333
Cardoso, B. M., Neto, Barbosa, L. P., Aguiar, C. S., Souza, R. S., Santana, A. L. A., Mendes, C. S.,... Leite, M. C. P. (2012). Follicle-stimulating hormone to substitute equine chorionic gonadotropin in the synchronization of ovulation in Santa Inês ewes. Revista Brasileira de Zootecnia, 41(3), 603-606. doi: 10.1590/S1516-35982012000300018
Cardoso, R. C., Barbosa, L. P., Souza, R. S., França, C. S., Ribeiro, M. D. M., Jr., Santana, A. L. A.,... Santos, R. S. (2018). Application of hormonal subdoses at acupoint Hou Hai in estrus synchronization protocols of goats. Semina: Ciências Agrárias, 39(3), 1135-1142. doi: 10.5433/1679-0359.2018 v39n3 p1135
Cavalcanti, A. S., Brandão, F. Z., Nogueira, L. A. G., & Fonseca, J. F. (2012). Effects of GnRH administration on ovulation and fertility in ewes subjected to estrous synchronization. Revista Brasileira de Zootecnia, 41(6), 1412-1418. doi: 10.1590/S1516-35982012000600014
Fonseca, J. F., Torres, C. A. A., Maffili, V. V., Prosperi, C. P., Santos, A. D. F., Borges, A. M.,... Rubert, M. (2003). Indução hormonal de estro em cabras nulíparas na estação de anestro. Revista Brasileira de Reprodução Animal, 27, 528-530.
Gardón, J. C., Escribano, B., Astiz, S., & Ruiz, S. (2015). Synchronization protocols in Spanish Merino sheep: reduction in time to estrus by the addition of eCG to a progesterone-based estrus synchronization protocol. Annals of Animal Science, 15(2), 409-418. doi: 10.1515/aoas-2015-0003
Ginther, O. J., & Kot, K. (1994). Follicular dynamics during the ovulatory season in goats. Theriogenology, 42(6), 987-1001. doi: 10.1016/0093-691X(94)90121-X
Leyva, V., Buckrell, B. C., & Walton, J. S. (1998). Regulation of follicular and ovulation in ewes by exogenous progestagen. Theriogenology, 50(3), 395-416. doi: 10.1016/S0093-691X(98)00148-4
Lin, J. H., Chan, W. W., Wu, L. S. (2006). Acupuntura para tratar distúrbios reprodutivos. In: A. M. Schoen (Ed.), Acupuntura veterinária: da arte antiga à medicina moderna. (2a ed. pp. 258-264). São Paulo: Editora Roca, 2006.
Martinez, A. C., Oliveira, F. S., Cadamuro, V. A. H., Bonamigo, C. G., & Folador, M. (2007). Uso de baixa dose de cloprostenol sódico administrado pelo ponto Bai Hui de acunpuntura em ovelhas. Archivos Veterinary Science, 12(Supl.), 231-232.
Moreira, N. H., Brasil, O. O., Silva, J. P., & Ramos, A. F. (2014). Utilização de FSH:LH como alternativa para estimular o crescimento folicular na sincronização do estro ovino. Acta Scientiae Veterinariae, 42, 1-7.
Nogueira, E., Silva, M. R., Silva, J. C. B., Abreu, U. P. G., Anache, N. A., Silva, K. C.,... Rodrigues, W. B. (2019). Timed artificial insemination plus heat I: effect of estrus expression scores on pregnancy of cows subjected to progesterone-estradiol-based protocols. Animal, 13(10), 2313-2318. doi: 10.1017/S 1751731119000442
Prates, J. F., Brair, V. L., Maia, A. L. R. S., Souza-Fabjan, J. M. G., Brandão, F. Z., Bonato, G. C., & Fonseca, J. F. (2019). Use of human intravaginal tampon embedded with natural progesterone induces synchronous estrus in Santa Inês ewes. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 71(1), 345-348. doi: 10-1590/1678-4162-10241
Scognamillo-Szabó, M. V. R., & Bechara, G. H. (2010). Acupuncture: history, basic principles and its use in veterinary medicine. Ciência Rural, 40(2), 1-13. doi: 10.1590/S0103-84782010005000004
Seekallu, S. V., Toosi, B. M., Duggavathi, R., Barrett, D. M. W., Davies, K. L., Waldner, C., & Rawlings, N. C. (2010). Ovarian antral follicular dynamics in sheep revisited: Comparison among estrous cycles with three or four follicular waves. Theriogenology, 73(5), 670-680. doi: 10.1016/j.theriogenology.2009.11. 007
Souza, D. O., Araujo, M. L., Biscarde, C. E. A., Mendes, C. S., Silva, M. A. A., Romero, D. C. M. R.,... Barbosa, L. P. (2019). Use of hormonal subdoses applied in Bai Hui acupuncture in estrus synchronization protocols for goats. Semina: Ciências Agrárias, 40(4), 1501-1512. doi: 10.5433/1679-0359.2019v40n4p1501
Teixeira, T. A., Fonseca, J. F., Souza-Fabjan, J. M. G., Carvalheira, L. R., Fernandes, D. A. M., & Brandão, F. Z. (2016). Efficiency of different hormonal treatments for estrus synchronization in tropical Santa Inês sheep. Tropical Animal Health Production, 48(3), 545-551. doi: 10.1007/s11250-015-0989-y
Vilariño, M., Cuadro, F., Santos, P. C., Neto, García-Pintos, C., & Menchaca, A. (2017). Time of ovulation and pregnancy outcomes obtained with the prostaglandina-based protocol Synchrovine for FTAI in sheep. Theriogenology, 90(1), 163-168. doi: 10.1016/j.theriogenology.2016.12.003
Wildeus, S, (2000). Current concepts in synchronization of estrus: Sheep and goats. Journal of Animal Science, 77(Supl.), 1-14. doi: 10.2527/jas2000.00218812007700ES0040x
Araújo, R. C. S. A., Barbosa, L. P., Souza, R. S., Santana, A. L. A., Carneiro, I. M. B., Machado, W. M.,... Jesus, R. D. L. (2019). Farmacopuntura utilizando os acupontos Hou Hai e Bai Hui em protocolos de sincronização de estro em cabras. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 71(2), 529-537. doi: 10.1590/1678-4162-10333
Cardoso, B. M., Neto, Barbosa, L. P., Aguiar, C. S., Souza, R. S., Santana, A. L. A., Mendes, C. S.,... Leite, M. C. P. (2012). Follicle-stimulating hormone to substitute equine chorionic gonadotropin in the synchronization of ovulation in Santa Inês ewes. Revista Brasileira de Zootecnia, 41(3), 603-606. doi: 10.1590/S1516-35982012000300018
Cardoso, R. C., Barbosa, L. P., Souza, R. S., França, C. S., Ribeiro, M. D. M., Jr., Santana, A. L. A.,... Santos, R. S. (2018). Application of hormonal subdoses at acupoint Hou Hai in estrus synchronization protocols of goats. Semina: Ciências Agrárias, 39(3), 1135-1142. doi: 10.5433/1679-0359.2018 v39n3 p1135
Cavalcanti, A. S., Brandão, F. Z., Nogueira, L. A. G., & Fonseca, J. F. (2012). Effects of GnRH administration on ovulation and fertility in ewes subjected to estrous synchronization. Revista Brasileira de Zootecnia, 41(6), 1412-1418. doi: 10.1590/S1516-35982012000600014
Fonseca, J. F., Torres, C. A. A., Maffili, V. V., Prosperi, C. P., Santos, A. D. F., Borges, A. M.,... Rubert, M. (2003). Indução hormonal de estro em cabras nulíparas na estação de anestro. Revista Brasileira de Reprodução Animal, 27, 528-530.
Gardón, J. C., Escribano, B., Astiz, S., & Ruiz, S. (2015). Synchronization protocols in Spanish Merino sheep: reduction in time to estrus by the addition of eCG to a progesterone-based estrus synchronization protocol. Annals of Animal Science, 15(2), 409-418. doi: 10.1515/aoas-2015-0003
Ginther, O. J., & Kot, K. (1994). Follicular dynamics during the ovulatory season in goats. Theriogenology, 42(6), 987-1001. doi: 10.1016/0093-691X(94)90121-X
Leyva, V., Buckrell, B. C., & Walton, J. S. (1998). Regulation of follicular and ovulation in ewes by exogenous progestagen. Theriogenology, 50(3), 395-416. doi: 10.1016/S0093-691X(98)00148-4
Lin, J. H., Chan, W. W., Wu, L. S. (2006). Acupuntura para tratar distúrbios reprodutivos. In: A. M. Schoen (Ed.), Acupuntura veterinária: da arte antiga à medicina moderna. (2a ed. pp. 258-264). São Paulo: Editora Roca, 2006.
Martinez, A. C., Oliveira, F. S., Cadamuro, V. A. H., Bonamigo, C. G., & Folador, M. (2007). Uso de baixa dose de cloprostenol sódico administrado pelo ponto Bai Hui de acunpuntura em ovelhas. Archivos Veterinary Science, 12(Supl.), 231-232.
Moreira, N. H., Brasil, O. O., Silva, J. P., & Ramos, A. F. (2014). Utilização de FSH:LH como alternativa para estimular o crescimento folicular na sincronização do estro ovino. Acta Scientiae Veterinariae, 42, 1-7.
Nogueira, E., Silva, M. R., Silva, J. C. B., Abreu, U. P. G., Anache, N. A., Silva, K. C.,... Rodrigues, W. B. (2019). Timed artificial insemination plus heat I: effect of estrus expression scores on pregnancy of cows subjected to progesterone-estradiol-based protocols. Animal, 13(10), 2313-2318. doi: 10.1017/S 1751731119000442
Prates, J. F., Brair, V. L., Maia, A. L. R. S., Souza-Fabjan, J. M. G., Brandão, F. Z., Bonato, G. C., & Fonseca, J. F. (2019). Use of human intravaginal tampon embedded with natural progesterone induces synchronous estrus in Santa Inês ewes. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 71(1), 345-348. doi: 10-1590/1678-4162-10241
Scognamillo-Szabó, M. V. R., & Bechara, G. H. (2010). Acupuncture: history, basic principles and its use in veterinary medicine. Ciência Rural, 40(2), 1-13. doi: 10.1590/S0103-84782010005000004
Seekallu, S. V., Toosi, B. M., Duggavathi, R., Barrett, D. M. W., Davies, K. L., Waldner, C., & Rawlings, N. C. (2010). Ovarian antral follicular dynamics in sheep revisited: Comparison among estrous cycles with three or four follicular waves. Theriogenology, 73(5), 670-680. doi: 10.1016/j.theriogenology.2009.11. 007
Souza, D. O., Araujo, M. L., Biscarde, C. E. A., Mendes, C. S., Silva, M. A. A., Romero, D. C. M. R.,... Barbosa, L. P. (2019). Use of hormonal subdoses applied in Bai Hui acupuncture in estrus synchronization protocols for goats. Semina: Ciências Agrárias, 40(4), 1501-1512. doi: 10.5433/1679-0359.2019v40n4p1501
Teixeira, T. A., Fonseca, J. F., Souza-Fabjan, J. M. G., Carvalheira, L. R., Fernandes, D. A. M., & Brandão, F. Z. (2016). Efficiency of different hormonal treatments for estrus synchronization in tropical Santa Inês sheep. Tropical Animal Health Production, 48(3), 545-551. doi: 10.1007/s11250-015-0989-y
Vilariño, M., Cuadro, F., Santos, P. C., Neto, García-Pintos, C., & Menchaca, A. (2017). Time of ovulation and pregnancy outcomes obtained with the prostaglandina-based protocol Synchrovine for FTAI in sheep. Theriogenology, 90(1), 163-168. doi: 10.1016/j.theriogenology.2016.12.003
Wildeus, S, (2000). Current concepts in synchronization of estrus: Sheep and goats. Journal of Animal Science, 77(Supl.), 1-14. doi: 10.2527/jas2000.00218812007700ES0040x
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