Physicochemical and colorimetric characterization of cryoconcentrated grape juices and their impact on Saccharomyces cerevisiae fermentation

Authors

DOI:

https://doi.org/10.5433/1679-0359.2026v47n1p235

Keywords:

Grape, Yeast, Cryoconcentration, Alcoholic fermentation, Freeze concentration.

Abstract

Cryoconcentration increases sugar concentration in grape juice, influencing alcoholic fermentation. Thus, physicochemical and colorimetric parameters of cryoconcentrated grape juices and their impact on alcoholic fermentation were evaluated. Five treatments, each with a volume of 1.5 L, were prepared. Treatments received 0.05 g L-1 potassium metabisulfite and 0.60 g L-1 Saccharomyces cerevisiae. Subsequently, treatments were incubated at 25.0 °C ± 2.0 °C and remained under these conditions until alcoholic fermentation was completed after 10 days (treatment 1), 18 days (treatments 2, 3, and 5), and 26 days (treatment 4). Alcoholic degree and residual reducing sugar levels were 10.56% ± 0.01% and 4.22 ± 0.42 g L-1 (treatment 1), 14.58% ± 0.03% and 34.53 ± 0.20 g L-1 (treatment 2), 14.91% ± 0.03% and 35.88 ± 0.56 g L-1 (treatment 3), 10.37% ± 0.03% and 76.03 ± 1.55 g L-1 (treatment 4), and 5.26% ± 0.02% and 208.50 ± 5.57 g L-1 (treatment 5), respectively. Total polyphenol content and total titratable acidity increased with higher volumes of cryoconcentrate in treatments. However, pH, volatile acidity, and phenolic profile did not show the same behavior. Cryoconcentration did not affect blue color or hue, but increased color intensity while reducing luminosity and red coloration. High concentrations of total soluble solids reduced the fermentative capacity of Saccharomyces cerevisiae in treatments 2, 3, 4, and 5, delaying fermentation and resulting in high residual sugar levels.

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Author Biographies

Fábio Martins Campos, Instituto Federal do Paraná

Laboratory Technician/Chemistry, Instituto Federal do Paraná, IFPR, Jaguariaíva, PR, Brazil.

Valter de Oliveira Souto, Universidade Estadual de Ponta Grossa

Doctoral student in the Postgraduate Program in Food Science and Technology, Food Engineering Department, UEPG, Ponta Grossa, PR, Brazil.

Gabriela Sperotto, Universidade Estadual de Ponta Grossa

Doctoral student in the Postgraduate Program in Food Science and Technology, Food Engineering Department, UEPG, Ponta Grossa, PR, Brazil.

Juliane Barreto de Oliveira, Universidade Federal do Rio Grande do Sul

Profa. Ph.D., Instituto Federal do Rio Grande do Sul, IFRS, Bento Gonçalves, RS, Brazil.

Celso Guarani Ruiz de Oliveira, Brazilian Agricultural Research Corporation

Analyst M.Sc., Empresa Brasileira de Pesquisa Agropecuária, EMBRAPA Uva e Vinho, Bento Gonçalves, RS, Brazil.

Juliana Fronza, Universidade Federal do Rio Grande do Sul

Profa. Me., Instituto Federal do Rio Grande do Sul (IFRS), Bento Gonçalves, RS, Brasil.

Giuliano Elias Pereira, Brazilian Agricultural Research Corporation

 Researcher Ph.D., EMBRAPA Uva e Vinho, Bento Gonçalves, RS, Brazil.

Marcelo Lazzarotto, Brazilian Agricultural Research Corporation

 Researcher Ph.D., EMBRAPA Uva e Vinho, Bento Gonçalves, RS, Brazil.

References

Adorno, W. T., Rezzadori, K., Arend, G. D., Chaves, V. C., Reginatto, F. H., Di Luccio, M., & Petrus, J. C. C. (2017). Enhancement of phenolic compounds content and antioxidant activity of strawberry (Fragaria × ananassa) juice by block freeze concentration technology. International Journal of Food Science and Technology, 52(3), 1-7. doi: 10.1111/ijfs.13335

Aider, M., & Halleux, D. (2009a). Cryoconcentration technology in the bio-food industry: principles and applications. LWT - Food Science and Technology, 42(3), 679-685. doi: 10.1016/j.lwt.2008.08.013

Aider, M., & Ounis, W. B. (2012). Skim milk cryoconcentration as affected by the thawing mode: gravitational vs. microwave-assisted. International Journal of Food Science and Technology, 47(1), 195-202. doi: 10.1111/j.1365-2621.2011.02826.x

Aider, M., Halleux, D., & Melnikova, I. (2009b). Skim acidic milk whey cryoconcentration and assessment of its functional properties: impact of processing conditions. Innovative Food Science and Emerging Technologies, 10(3), 334-341. doi: 10.1016/j.ifset.2009.01.005

Amran, N. A., Samsuri, S., Safiei, N. Z., Zakaria, Z. Y., & Jusoh M. (2016). Review: parametric study on the performance of progressive cryoconcentration system. Chemical Engineering Communications, 203:957-975. doi:10.1080/00986445.2015.1075982

Belén, F., Sánchez, J., Hernández, E., Auleda, J. M., & Raventós, M. (2012). One option for the management of wastewater from tofu production: Freeze concentration in a falling-film system. Journal of Food Engineering, 110(3), 364-373. doi: 10.1016/j.jfoodeng.2011.12.036

Benedetti, S., Prudêncio, E. S., Nunes, G. L., Guizoni, K., Fogaça, L. A., & Petrus, J. C. C. (2015). Antioxidant properties of tofu whey concentrate by freeze concentration and nanofiltration processes. Journal of Food Engineering, 160(2015), 49-55. doi: 10.1016/j.jfoodeng.2015.03.021

Boaventura, B. C. B., Murakami, A. N. N., Prudêncio, E. S., Maraschin, M., Murakami, F. S., Amante, E. R., & Amboni, R. D. M. C. (2013). Enhancement of bioactive compounds content and antioxidant activity of aqueous extract of mate (Ilex paraguariensis A. St. Hil.) through freeze concentration technology. Food Research International, 53(2), 686-692. doi: 10.1016/j.foodres.2012.07.04

Bredun, M. A., Prestes, A. A., Panceri, C. P., Prudêncio, E. S., & Burin, V. M. (2023). Bioactive compounds recovery by freeze concentration process from winemaking by-product. Food Research International, 173(1), 113220. doi: 10.1016/j.foodres.2023.113220

Cardoso, A. D. (2019). O vinho: da uva à garrafa (2a ed.) Agrobook.

Casas-Forero, N., Orellana-Palma, P., & Petzold, G. (2021). Recovery of solutes from ice and concentrated fractions in centrifugal block cryoconcentration applied to blueberry juice. Food Bioprocess Technology, 14(3), 1155-1168. doi: 10.1590/fst.29819

Cochachin-Carrera, B., Moreno-Cuevas, J., & Carvajal-Mena, N. (2023). Effects of concentration by block freezing and vacuum evaporation on the physicochemical properties and digestibility of whey. CyTA - Journal of Food, 21(1), 313-320. doi: 10.1080/19476337.2023.2196321

Dunaway, K. W., Dasari, R. K., Bennett, N. G., & Berson, R. E. (2010). Characterization of changes in viscosity and insoluble solids content during enzymatic saccharification of pretreated corn stover slurries. Bioresource Technology, 101(10), 3575-3582. doi: 10.1016/j.biortech.2009.12.07 1

Geraldine, J. M. S., Murugesan, G., Chellan, R., & Asit, M. B. (2012). Osmotic shock augments ethanol stress in Saccharomyces cerevisiae MTCC 2918. Current Microbiology, 64(2), 100-105. doi: 10.1007/s00284-011-0036-9

Gil, N., Quinteros, G., Blanco, M., Samsuri, S., Amran, N. A., Orellana-Palma, P., Schwinden, E., & Hernández, E. (2023). Vacuum-assisted block freeze concentration studies in cheese whey and its potential in lactose recovery. Foods, 12(4), 836. doi: 10.3390/foods12040836

Haas, I. C. S., Espindola, J. S., Liz, G. R., Luna, A. S., Bordignon-Luiz, M. T., Prudêncio, L. S., Gois, J. S., & Fedrigo, I. M. T. (2022). Gravitational assisted three-stage block freeze concentration process for producing enriched concentrated orange juice (Citrus sinensis L.), Multi-elemental profiling and polyphenolic bioactives. Journal of Food Engineering, 315(1), 110802. doi: 10.1016/j.jfoodeng.2021.110802

Hernández, E., Raventós, M., Auleda, J. M., & Ibarz, A. (2010). Freeze concentration of must in a pilot plant falling film cryoconcentrator. Innovative Food Science and Emerging Technology, 11(1), 130-136. doi: 10.1016/j.ifset.2009.08.014

Hwang, S.-W., & Park, H.-D. (2009). Characteristics of red wine fermentation of freeze-concentrated Campbell Early grape juice using various wine yeasts. Korean Journal of Food Preservation, 16(6), 977-984. https://koreascience.kr/article/JAKO200911764904721.pdf

Hwang, S.-W., Hong, J.-A., & Park, H.-D. (2011). Characteristics of ice wine fermentation of freeze-concentrated Campbell Early grape juice by S. cerevisiae S13 and D8 isolated from korean grapes. Korean Journal of Food Preservation, 18(5), 811-816. doi: 10.11002/kjfp.2011.18.5.811

International Organization of Vine and Wine (2022). Compendium of international methods of wine and must analysis. Edition Paris: OIV. https://www.oiv.int/standards/compendium-of-international-methods-of-wine-and-must-analysis

International Organization of Vine and Wine 2023. (2024). State of the world vine and Wine sector in 2023. Disponível em: https://www.oiv.int/....

Lazzarotto, S. R. S., Lazzarotto, M., Silveira, A. C., Wendling, I., & Schnitzler, E. (2021). Corn starch incorporated with freeze-concentrated Ilex paraguariensis extracts: a potential nutraceutical product. Journal of Termal Analysis and Calorimetry, 141(1), 1-6. doi: 10.1007/s10973-020-09971-7

Marafon, K., Pereira-Coelho, M., Haas, I. C. S., Wanderley, B. R. S. M., Gois, J. S., Vitali, L., Luna, A. S., Canella, M. H. M., Hernández, E., Amboni, R. D. M. C., Prudencio, E. S. (2024). An opportunity for acerola pulp (Malpighia emarginata DC) valorization evaluating its performance during the block cryoconcentration by physicochemical, bioactive compounds, HPLC–ESI-MS/MS, and multi-elemental profile analysis. Food Research International, 176(2024), 113793. doi: 10.1016/j.foodres.2023.113793

Meneses, D. L., Ruiz, Y., Hernández, E., & Moreno, F. L. (2021). Multi-stage block freeze-concentration of green tea (Camellia sinensis) extract. Journal of Food Engineering, 209(2021), 110381. doi: 10.1016/j.jfoodeng.2020.110381

Miyawaki, O., & Inakuma, T. (2021a). Development of progressive freeze concentration and its application: a review. Food and Bioprocess Technology, 14(1), 39-51. doi: 10.1007/s11947-020-02517-7

Miyawaki, O., Chiaki Omote, C., Koyanagi, T., Sasaki, T., Take, H., Matsuda, A., Tadokoro, K., & Miwa, S. (2021b). Interfacial freeze concentration of Ruby Roman grape juice and its application to winemaking. Journal of the Japanese Society for Food Science and Technology, 68(4), 159-165. doi: 10.3136/nskkk.68.159

Miyawaki, O., Omote, C., Koyanagi, T., Sasaki, T., Take, H., Matsuda, A., Tadokoro, K., & Miwa, S. (2020). Progressive freeze-concentration of ‘Kyoho’ grape (Vitis Labruscana Bailey) juice and its application to wine making. Food Engineering, 21(4), 171-177. doi: 10.11301/jsfe.2058 1

Moreno, F. L., Hernández, E., Raventós, M., & Ruiz, Y. (2014b). Block freeze-concentration of coffee extract: effect of freezing and thawing stages on solute recovery and bioactive compounds. Journal of Food Engineering, 120(2014), 158-166. doi: 10.1016/j.jfoodeng.2013.07.034

Moreno, F. L., Hernández, E., Raventós, M., Robles, C., & Ruiz, Y. (2014a). A process to concentrate coffee extract by the integration of falling film and block freeze-concentration. Journal of Food Engineering, 128(2014), 88-95. doi: 10.1016/j.jfoodeng.2013.12.022

Nakagawa, K., Maebashi, S., & Maeda, K. (2010). Freeze-thawing as a path to concentrate aqueous solution. Separation and Purification Technology, 73(3), 403-408. doi: 10.1016/j.seppur.2010.04. 031

Orellana-Palma, P., Petzold, G., Pierre, L., & Pensaben, J. M. (2017). Protection of polyphenols in blueberry juice by vacuum-assisted block freeze concentration. Food and Chemical Toxicology, 109(2), 1093-1102. doi: 10.1016/j.fct.2017.03.038

Orellana-Palma, P., Petzold, G., Torres, N., & Aguilera, M. (2018). Elaboration of orange juice concentrated by vacuum-assisted block freeze concentration. Journal of Food Processing and Preservation, 42(2), e13438. doi: 10.1111/jfpp.13438

Osorio, M., Moreno, F. L., Raventós, M., Hernández, E., & Ruiz, Y. (2018). Progressive stirred freeze-concentration of ethanol-water solutions. Journal of Food Engineering, 224(2018), 71-79. doi: 10.1016/j.jfoodeng.2017.12.026

Park, H., Park, E. K., Choi, S., Han, B., Noh, J., & Park, J.-M. (2019). Quality characteristics and volatile flavor compounds of doonuri wine using freeze concentration. Korean Journal Food and Nutrition, 32(5), 485-493. doi: 10.9799/ksfan.2019.32.5.485

Park, H., Park, E. K., Choi, S., Shin, H., Kim, M.-J., & Park, J.-M. (2020). Quality characteristics of cheong-soo grape wine by freeze concentration fermented with different yeasts. Korean Journal Food and Nutrition, 33(5), 512-523. doi: 10.9799/ksfan.2020.33.5.512

Petzold, G., Moreno, J., Lastra, P., Rojas, K., & Orellana, P. (2015). Block freeze concentration assisted by centrifugation applied to blueberry and pineapple juices. Innovative Food Science and Emerging Technologies, 30(2015), 192-197. doi: 10.1016/j.ifset.2015.03.007

Petzold, G., Niranjan, K., & Aguilera, J. M. (2013). Vacuum-assisted freeze concentration of sucrose solutions. Journal of Food Engineering, 115(3), 357-361. doi: 10.1016/j.jfoodeng.2012. 10.048

Petzold, G., Orellana, P., Moreno, J., Cerda, E., & Parra, P. (2016). Vacuum-assisted block freeze concentration applied to wine. Innovative Food Science and Emerging Technologies, 36(2016), 330-335. doi: 10.1016/j.ifset. 2016.07.019

Ribeiro, M. C., Santos, A., Riachi, L. G., Rodrigues, A. C. B., Coelho, G. C., Marcellini, P. S., Bento, C. A. M., & Maria, C. A. B. de. (2017). The effects of roasted yerba mate (Ilex paraguariensis A. ST. Hil.) consumption on glycemia and total serum creatine phosphokinase in patients with traumatic brain injury. Journal of Functional Foods, 28(2017), 240-245. doi: 10.1016/j.jff.2016.11.024

Safiei, N. Z., Ngadi, N., Johari, A., Zakaria, Z. Y., & Jusoh, M. (2017). Grape juice concentration by progressive freeze concentrator sequence system. Journal of Food Processing and Preservation, 41(1), e12910. doi: 10.1111/jfpp.12910

Sánchez, J., Ruiz, Y., Auleda, J. M., Hernández, E., & Raventós, M. (2009). Freeze concentration in the fruit juices industry. Food Science and Technology International, 15(4), 303-315. doi: 10.1177/1082013209344267

Sun, H.-H., Ma, H.-Q., & Chen, S.-W. (2007). Effects on qualities of lower sugar grape must and wine with freeze concentration technology. Food Science, 28(5), 86-89. https://www.spkx.net.cn/ EN/

Vavřiník, A., Štusková, K., Baroň, M., & Sochor, J. (2022). The production of wine vinegar using different types of acetic acid bacteria. Potravinarstvo Slovak Journal of Food Sciences, 16(2022), 556-567. doi: 10.5219/1723

Vuist, J.-E., Boom, R. M., & Schutyser, M. A. I. (2021). Solute inclusion and freezing rate during progressive freeze concentration of sucrose and maltodextrin solutions. Drying Technology, 39(10), 1285-1293. doi: 10.1080/07373937.2020.1742151

Wu, Y.-Y., Xing, K., Zhang, X.-X., Wang, H., Wang, Y., Wang, F., & Li, J.-M. (2017). Influence of freeze concentration technique on aromatic and phenolic compounds, color attributes, and sensory properties of Cabernet Sauvignon wine. Molecules, 22(6), 899-917. doi: 10.33 90/molecu les22060899

Zhang, Q., Sun, X., Sheng, Q., Chen, J., Huang, W., & Zhan, J. (2016). Effect of suspension freeze-concentration technology on the quality of wine. South African Journal of Enology and Viticulture, 37(1), 39-46 (2016). doi: 10.21548/37-1-757

Zielinski, A. A., Zardo, D. M., Alberti, A., Bortolini, D. G., Benvenutti, L., Demiate, I. M., & Nogueira, A. (2019). Effect of cryoconcentration process on phenolic compounds and antioxidant activity in apple juice. Journal of the Science of Food and Agriculture, 99(6), 2786-2792. doi: 10.1002/jsfa.9486

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Published

2026-06-19

How to Cite

Campos, F. M., Souto, V. . de O., Sperotto, G., Oliveira, J. B. de, Oliveira, C. G. R. de, Fronza, J., … Lazzarotto, M. (2026). Physicochemical and colorimetric characterization of cryoconcentrated grape juices and their impact on Saccharomyces cerevisiae fermentation. Semina: Ciências Agrárias, 47(1), 235–254. https://doi.org/10.5433/1679-0359.2026v47n1p235

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