Protein analysis of polymorphonuclear leukocytes from domestic cats
DOI:
https://doi.org/10.5433/1679-0359.2024v45n6p1873Keywords:
Felis catus, Granules, Neutrophils, Proteomics.Abstract
Neutrophils are the first line of defense against microorganisms, making them a crucial component of innate immunity. This study aimed to characterize the neutrophil proteome of Felis catus. Granulocytes were isolated from cat blood and purified, and 1 × 107 cells were used to identify proteins. In total, 102 neutrophil proteins from cats were identified and categorized on the basis of their abundance. Proteoglycan-3, which is involved in various biological processes, including neutrophil activation and the immune response, was the most abundant protein. Among the metabolic pathways, glycolysis involved the largest group of identified proteins (nine proteins). Proteins involved in molecular functions, cellular components, and biological processes were also identified. The identification of proteins in this study is expected to aid future research by elucidating certain characteristics of the species, identifying potential markers or drugs, and facilitating rapid, non-invasive diagnosis of diseases that particularly affect felines.
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