Evaluation of the quality characteristics of chilled tilapia fillets subjected to different levels of pre-slaughter stress
DOI:
https://doi.org/10.5433/1679-0359.2025v46n5p1389Keywords:
Fillet, Fish stress, Oreochromis niloticus, Processing.Abstract
The objective of this study was to evaluate the effect of three stress levels, minimal (MS), short-term (SS), and long-term (LS), on the quality of Nile tilapia fillets during refrigerated storage. In the MS treatment, fish were maintained at a density of 60 kg m-³ for 24 hr, whereas in the SS and LS treatments, fish were kept at a density of 300 kg m-³ for 1 hr (SS) or 24 hr (LS), respectively. Serum cortisol levels were measured, and meat pH, color, and tenderness were assessed for 10 fish per treatment 0, 1, 2, 4, and 8 post-mortem days. The highest serum cortisol level was found in LS fish, while the lowest average was observed in MS fish. The pH was higher in LS fish. Fillets from MS fish had greater tenderness at 8 days post-mortem. The color development of stressed fish showed higher lightness on days 0, 2, and 8 post-mortem, and red intensity was lower in MS fish. No significant differences were observed in the yellow intensity of the fillets. It is concluded that short- and long-term crowding stress results in higher stress levels, leading to alterations in the development of pH, texture, and coloration of Nile tilapia fillets.
Downloads
References
Almeida, T. P. O., Fuchs, R. H. B., Droval, A. A., Marques, L. L. M., & Cardoso, F. A. R. (2022). Study on antemortem stress and seasonal influence to mitigate the incidence of disaggregated fillets in the fish slaughtering industry. Journal of Food Processing and Preservation, 46(11), e16943. doi: 10.1111/jfpp.16943 DOI: https://doi.org/10.1111/jfpp.16943
Anders, N., Eide, I., Lerfall, J., Roth, B., & Breen, M. (2020). Physiological and flesh quality consequences of pre-mortem crowding stress in Atlantic mackerel (Scomber scombrus). PLoS One, 15(2), e0228454. doi: 10.1371/journal.pone.0228454 DOI: https://doi.org/10.1371/journal.pone.0228454
Bahuaud, D., Mørkøre, T., Østbye, T. K., Veiseth-Kent, E., Thomassen, M. S., & Ofstad, R. (2010). Muscle structure responses and lysosomal cathepsins B and L in farmed Atlantic salmon (Salmo salar L.) pre-and post-rigor fillets exposed to short and long-term crowding stress. Food Chemistry, 118(3), 602-615. doi: 10.1016/j.foodchem.2009.05.028 DOI: https://doi.org/10.1016/j.foodchem.2009.05.028
Bai, C., Qi, X., Wang, Z., Wang, J., Qiu, L., Li, H., Zu, X., Li, H., Xiong, G., & Liao, T. (2024). Effect of density stress on the physiological, biochemical, and immunological parameters of juvenile Pelteobagrus fulvidraco during simulated transportation. Aquaculture Reports, 34(2024), 101911. doi: 10.1016/j.aqrep.2023.101911 DOI: https://doi.org/10.1016/j.aqrep.2023.101911
Banhara, D. G. A., Mendonça, W. C. B., Goes, E. S. R., Goes, M. D., Braz, P. H., & Honorato, C. A. (2021). Effect of different stocking densities on pre-slaughter stress based on respiratory parameters in Nile tilapia (Oreochromis niloticus). Pan-American Journal of Aquatic Sciences, 16(3), 270-275. doi: 10.54451/PanamJAS.16.3.270 DOI: https://doi.org/10.54451/PanamJAS.16.3.270
Cheng, J. H., Sun, D. W., Zeng, X. A., & Liu, D. (2015). Recent advances in methods and techniques for freshness quality determination and evaluation of fish and fish fillets: a review. Critical Reviews in Food Science and Nutrition, 55(7), 1012-1225. doi: 10.1080/10408398.2013.769934 DOI: https://doi.org/10.1080/10408398.2013.769934
Daskalova, A. (2019). Farmed fish welfare: stress, post-mortem muscle metabolism, and stress related meat quality changes. International Aquatic Research, 11(2), 113-124. doi: 10.1007/s40071-019-0230-0 DOI: https://doi.org/10.1007/s40071-019-0230-0
Delphino, M. K., Leal, C. A., Gardner, I. A., Assis, G. B., Roriz, G. D., Ferreira, F., Figueiredo, H. C. P., & Gonçalves, V. S. (2019). Seasonal dynamics of bacterial pathogens of Nile tilapia farmed in a Brazilian reservoir. Aquaculture, 498(2019), 100-108. doi: 10.1016/j.aquaculture.2018.08.023 DOI: https://doi.org/10.1016/j.aquaculture.2018.08.023
Food and Agriculture Organization of the United Nations (2024). The state of world fisheries and aquaculture 2024: Blue transformation in action. Rome: Food and Agriculture Organization of the United Nations. doi: 10.4060/cd0683en DOI: https://doi.org/10.4060/cd0683en
Goes, E. S. D. R., Goes, M. D., Castro, P. L. D., Lara, J. A. F. D., Vital, A. C. P., & Ribeiro, R. P. (2019). Imbalance of the redox system and quality of tilapia fillets subjected to pre-slaughter stress. PloS One, 14(1), e0210742. doi: 10.1371/journal.pone.0210742 DOI: https://doi.org/10.1371/journal.pone.0210742
Kayan, A., Boontan, I., Jaturssitha, S., Wicke, M., & Kreuzer, M. (2015). Effect of slaughter weight on meat quality of Nile Tilapia (Oreochromis niloticus). Agriculture and Agricultural Science Procedia, 5(2015), 159-163. doi: 10.1016/j.aaspro.2015.08.024 DOI: https://doi.org/10.1016/j.aaspro.2015.08.024
Kumar, G., & Engle, C. R. (2016). Technological advances that led to growth of shrimp, salmon, and tilapia farming. Reviews in Fisheries Science & Aquaculture, 24(2), 136-152. doi: 10.1080/23308249.2015.1112357 DOI: https://doi.org/10.1080/23308249.2015.1112357
Leonard, J. N., & Skov, P. V. (2022). Capacity for thermal adaptation in Nile tilapia (Oreochromis niloticus): effects on oxygen uptake and ventilation. Journal of Thermal Biology, 105(2022), e103206. doi: 10.1016/j.jtherbio.2022.103206 DOI: https://doi.org/10.1016/j.jtherbio.2022.103206
Lerfall, J., Roth, B., Skare, E. F., Henriksen, A., Betten, T., Dziatkowiak-Stefaniak, M. A., & Rotabakk, B. T. (2015). Pre-mortem stress and the subsequent effect on flesh quality of pre-rigor filleted Atlantic salmon (Salmo salar L.) during ice storage. Food Chemistry, 175(2015), 157-165. doi: 10.1016/j.foodchem.2014.11.111 DOI: https://doi.org/10.1016/j.foodchem.2014.11.111
Li, W., Li, D., Yang, Q., Liu, L., Liu, J., Lu, J., Wang, Y., Tang, R., Li, L., & Zhang, X. (2023). Long-term crowding stress induces chronic inflammatory response and declines the immunity of grass carp (Ctenopharyngodon idella). Aquaculture, 577(2023), 739976. doi: 10.1016/j.aquaculture.2023.739976 DOI: https://doi.org/10.1016/j.aquaculture.2023.739976
Magalhães, C. R., Schrama, D., Farinha, A. P., Revets, D., Kuehn, A., Planchon, S., Rodrigues, P. M., & Cerqueira, M. (2020). Protein changes as robust signatures of fish chronic stress: a proteomics approach to fish welfare research. BMC Genomics, 21(309), 1-16. doi: 10.1186/s12864-020-6728-4 DOI: https://doi.org/10.1186/s12864-020-6728-4
Manduca, L. G., Silva, M. A., Alvarenga, E. R., Alves, G. F. O., Fernandes, A. F. A., Assumpcao, A. F., Cardoso, C. C., Sales, S. C. M., Teixeira, E. A., Silva, M. A., & Turra, E. M. (2020). Effects of a zero exchange biofloc system on the growth performance and health of Nile tilapia at different stocking densities. Aquaculture, 521(2020), 735064. doi: 10.1016/j.aquaculture.2020.735064 DOI: https://doi.org/10.1016/j.aquaculture.2020.735064
Mohamed, F. A., Khogali, F. A., Mohamed, A. H., Deng, O. O., & Mohammed, A. A. (2016). Body weight characteristics and chemical composition of Nile tilapia (Oreochromis niloticus) collected from three different Sudanese dams. International Journal of Fisheries and Aquatic Studies, 4(5), 507-510. https://www.fisheriesjournal.com/archives/2016/vol4issue5/PartG/4-5-28-405.pdf
PeixeBR (2025). Anuário 2025- Panorama da aquicultura brasileira. Associação Brasileira da Piscicultura. https://www.peixebr.com.br/anuario-2025/
Pongsetkul, J., Benjakul, S., Takeungwongtrakul, S., & Sai-Ut, S. (2022). Impact of stocking density during live transportation on meat quality of Nile tilapia (Oreochromis niloticus) and their changes during storage. Journal of Food Processing and Preservation, 46(5), 1-11. doi: 10.1111/jfpp.16523 DOI: https://doi.org/10.1111/jfpp.16523
Rahman, M. L., Shahjahan, M., & Ahmed, N. (2021). Tilapia farming in Bangladesh: adaptation to climate change. Sustainability, 13(14), 7657. doi: 10.3390/su13147657 DOI: https://doi.org/10.3390/su13147657
Rahmanifarah, K., Shabanpour, B., & Sattari, A. (2011). Effects of clove oil on behavior and flesh quality of common carp (Cyprinus carpio L.) in comparison with pre-slaughter CO2 stunning, chilling and asphyxia. Turkish Journal of Fisheries and Aquatic Sciences, 11(1), 139-147. doi: 10.4194/trjfas.2011.0118 DOI: https://doi.org/10.4194/trjfas.2011.0118
Sadoul, B., & Geffroy, B. (2019). Measuring cortisol, the major stress hormone in fishes. Journal of Fish Biology, 94(4), 540-555. doi: 10.1111/jfb.13904 DOI: https://doi.org/10.1111/jfb.13904
Santos, S. P., Silva, M. I., Godoy, A. C., Banhara, D. G. A., Goes, M. D., Goes, E. S. R., & Honorato, C. A. (2024). Respiratory and muscular effort during pre-slaughter stress affect Nile tilapia fillet quality. PloS One, 19(7), e0306880. doi: 10.1371/journal.pone.0306880 DOI: https://doi.org/10.1371/journal.pone.0306880
Skjervold, P. O., Fjæra, S. O., Østby, P. B., & Einen, O. (2001). Live-chilling and crowding stress before slaughter of Atlantic salmon (Salmo salar). Aquaculture, 192(2-4), 265-280. doi: 10.1016/S0044-8486(00)00447-6 DOI: https://doi.org/10.1016/S0044-8486(00)00447-6
Svalheim, R. A., Aas-Hansen, Ø., Heia, K., Karlsson-Drangsholt, A., Olsen, S. H., & Johnsen, H. K. (2020). Simulated trawling: Exhaustive swimming followed by extreme crowding as contributing reasons to variable fillet quality in trawl-caught Atlantic cod (Gadus morhua). PLoS One, 15(6), e0234059. doi: 10.1371/journal.pone.0234059 DOI: https://doi.org/10.1371/journal.pone.0234059
Valenti, W. C., Barros, H. P., Moraes-Valenti, P., Bueno, G. W., & Cavalli, R. O. (2021). Aquaculture in Brazil: past, present and future. Aquaculture Reports, 19(2021), 100611. doi: 10.1016/j.aqrep.2021.100611 DOI: https://doi.org/10.1016/j.aqrep.2021.100611
Yarahmadi, P., Miandare, H. K., Hoseinifar, S. H., Gheysvandi, N., & Akbarzadeh, A. (2015). The effects of stocking density on hemato-immunological and serum biochemical parameters of rainbow trout (Oncorhynchus mykiss). Aquaculture International, 23(2015), 55-63. doi: 10.1007/s10499-014-9797-z DOI: https://doi.org/10.1007/s10499-014-9797-z
Yousefi, M., Hoseini, S. M., Weber, R. A., Silva, E. da, Rajabiesterabadi, H., Arghideh, M., & Delavar, F. H. (2022). Alleviation of transportation-induced stress in Nile tilapia, Oreochromis niloticus, using brackish water. Aquaculture Reports, 27(2022), 101378. doi: 10.1016/j.aqrep.2022.101378 DOI: https://doi.org/10.1016/j.aqrep.2022.101378
Yue, G. H., Lin, H. R., & Li, J. L. (2016). Tilapia is the fish for next-generation aquaculture. International Journal of Marine Science and Ocean Technology, 3(1), 11-13. doi: 10.19070/2577-4395-160003 DOI: https://doi.org/10.19070/2577-4395-160003
Zhang, T., Zhang, L., Yin, T., You, J., Liu, R., Huang, Q., Shi, L., Wang, L., Liao, T., Wang, W., & Ma, H. (2023). Recent understanding of stress response on muscle quality of fish: from the perspective of industrial chain. Trends in Food Science & Technology, 140(2023), 104145. doi: 10.1016/j.tifs.2023.104145 DOI: https://doi.org/10.1016/j.tifs.2023.104145
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Elenice Souza dos Reis Goes, Maria Ildilene da Silva, Marcio Douglas Goes, Milton Teixeira de Moraes Junior, Valfredo Figueira da Silva, Ricardo Pereira Ribeiro

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Semina: Ciências Agrárias adopts the CC-BY-NC license for its publications, the copyright being held by the author, in cases of republication we recommend that authors indicate first publication in this journal.
This license allows you to copy and redistribute the material in any medium or format, remix, transform and develop the material, as long as it is not for commercial purposes. And due credit must be given to the creator.
The opinions expressed by the authors of the articles are their sole responsibility.
The magazine reserves the right to make normative, orthographic and grammatical changes to the originals in order to maintain the cultured standard of the language and the credibility of the vehicle. However, it will respect the writing style of the authors. Changes, corrections or suggestions of a conceptual nature will be sent to the authors when necessary.











