Palavras-chave
Trematódeo
Pequenos ruminantes
Imunomodulação
Citocina.
Pequenos ruminantes
Imunomodulação
Citocina.
Como Citar
Mendes, E. A., Pereira, C. A. de J., & Lima, W. dos S. (2021). Resposta imune em ovinos da raça Santa Inês durante a infecção por Fasciola hepatica. Semina: Ciências Agrárias, 42(6), 3289–3304. https://doi.org/10.5433/1679-0359.2021v42n6p3289
Resumo
No presente estudo, observou-se a resposta proliferativa e os perfis de expressão de citocinas IFN - y, IL-10, IL-4 no sangue periférico, e a produção de IgG, aos 60 e 210 dias após infecção (dpi) experimental de ovinos Santa Inês com 250 metacercárias de Fasciola hepatica. O perfil de expressão de citocinas aos 60 dpi foi caracterizado pela produção de IFN - y e IL-10, o que é indicativo de uma resposta mista inicial Th1/Th2. A modulação da resposta ocorreu aos 210 dpi, com predominância de IL-10 e IL-4 sobre IFN - y. As células mononucleares do sangue periférico estimuladas com antígeno total de F. hepatica adulta exibiram capacidade proliferativa aos 60 dpi, enquanto a resposta foi consistente com uma modulação de Th1 para Th2 na fase crônica. A resposta do anticorpo IgG foi maior aos 60 dpi do que aos 210 dpi. A modulação tardia da resposta dos linfócitos T em associação com a predominância de IL-10 e IL-4 aos 210 dpi sugere o envolvimento dessas citocinas após o estabelecimento dos parasitos nos ductos biliares. A transição de uma resposta mista para uma resposta com pefil regulador na fase crônica também foi acompanhada por redução dos ovos por grama de fezes.Referências
Aldridge, A., & O'Neill, S. M. (2016). Fasciola hepatica tegumental antigens induce anergic-like T cells via dendritic cells in a mannose receptor dependent manner. European Journal of Immunology, 46(5), 1180-1192. doi: 10.1002/eji.201545905
Beesley, N. J., Williams, D. J. L., Paterson, S., & Hodgkinson, J. (2017). Fasciola hepatica demonstrates high levels of genetic diversity, a lack of population structure and high gene flow: possible implications for drug resistance. International Journal for Parasitology, 47(1), 11-20. doi:10.1016/j.ijpara.2016.09.0 07
Boray, J. C. (1967). Studies on experimental infections with Fasciola hepatica, with particular reference to acute fascioliasis in sheep. Annals of Tropical Medicine & Parasitology, 61(4), 439-450. doi: 10.1080/0 0034983.1967.11686513
Chauvin, A., Bouvet, G., & Boulard, C. (1995). Humoral and cellular immune responses to Fasciola hepatica experimental primary and secondary infection in sheep. International Journal for Parasitology, 25(10), 1227-1241. doi: 10.1016/0020-7519(95)00039-5
Dalton, J. P., Robinson, M. W., Mulcahy, G., O'Neill, S. M., & Donnelly, S. (2013). Immunomodulatory molecules of Fasciola hepatica: candidates for both vaccine and immunotherapeutic development. Veterinary Parasitology, 195(3-4), 272-285. doi: 10.1016/j.vetpar.2013.04.
Dracz, R. M., & Lima, W. S. (2014). Autochthonous infection of buffaloes and cattle by Fasciola hepatica in Minas Gerais, Brazil. Revista Brasileira de Parasitologia Veterinária, 23(3), 413-416. doi: 10.1590/S1 984-29612014054
Dracz, R. M., Ribeiro, V. M. A., Pereira, C. A. J., & Lima, W. S. (2016). Ocorrência de Fasciola hepatica (Linnaeus, 1758) em capivara (Hydrochoerus hydrochaeris) (Linnaeus, 1766) em Minas Gerais, Brasil. Revista Brasileira de Parasitologia Veterinária, 25(3), 364-367. doi: 10.1590/S1984-29612016021
Flynn, R. J., & Mulcahy, G. (2008). The roles of IL-10 and TGF-beta in controlling IL-4 and IFN-gamma production during experimental Fasciola hepatica infection. International Journal for Parasitology, 38(14), 1673-1680. doi: 10.1016/j.ijpara.2008.05.008
Flynn, R. J., & Musah-Eroje, M. (2020). Evasion of Host Immunity during Fasciola hepatica Infection. In M. Cancela, & G. Maggioli (Eds.), Fasciola hepatica: Methods and Protocols, Methods in Molecular Biology. (Vol. 2137, pp. 107-115). Recovered from https://doi.org/10.1007/978-1-0716-0475-5_1,© Springer Science Business Media, LLC, part of Springer Nature 2020
Furst, T., Duthaler, U., Sripa, B., Utzinger, J., & Keiser, J. (2012). Trematode infections: liver and lung flukes. Infectious disease clinics, 26(2), 399-419. doi: 10.1016/j.idc.2012.03.008
Hacariz, O., Sayers, G., Flynn, R. J., Lejeune, A., & Mulcahy, G. (2009). IL-10 and TGF-beta1 are associated with variations in fluke burdens following experimental fasciolosis in sheep. Parasite Immunology, 31(10), 613-622. doi: 10.1111/j.1365-3024.2009.01135.x
Lima, W. dos S., Soares, L. R., Barcante, T. A., Guimaraes, M. P., & Barcante, J. M. (2009) Occurrence of Fasciola hepatica (Linnaeus, 1758) infection in Brazilian cattle of Minas Gerais, Brazil. Revista Brasileira de Parasitologia Veterinária, 18(2), 27-30. doi: 10.4322/rbpv.01802006
Mendes, E. A., Mendes, T. A. O., Santos, S. L., Menezes-Souza, D., Bartholomeu, D. C., Martins, I. V. F., Silva, L.M., Lima, W. S. (2013). Expression of IL-4, IL-10 and IFN-gamma in the liver tissue of cattle that are naturally infected with Fasciola hepatica. Veterinary Parasitology, 195(1-2), 177-182. doi: 10. 1016/j.vetpar.2013.03.035
Moreau, E., & Chauvin, A. (2010). Immunity against helminths: interactions with the host and the intercurrent infections. Journal of Biomedicine and Biotechnology, 2010 (428593), 1-10. doi: 10.1155/ 2010/428593
Moreau, E., Herve, S., Yu, Z. W., & Alain, C. (2002). Modulation of sheep lymphocyte responses by Fasciola hepatica excretory-secretory products. Veterinary Parasitology, 108(3), 207-215. doi: 10. 1016/S0304-4017(02)00196-6
Moxon, J. V., Flynn, R. J., Golden, O., Hamilton, J. V., Mulcahy, G., & Brophy, P. M. (2010). Immune responses directed at egg proteins during experimental infection with the liver fluke Fasciola hepatica. Parasite Immunology, 32(2), 111-124. doi: 10.1111/j.1365-3024.2009.01171.x
Pereira, C. A. J., Oliveira, L. L. S., Coaglio, A. L., Santos, F. S. O., Cezar, R. S. M., Mendes, T., Oliveira F. L. P., Consenza, G. Lima, W. S. (2016). Anti-helminthic activity of Momordica charantia L. against Fasciola hepatica eggs after twelve days of incubation in vitro. Veterinary Parasitology, 228(15), 160-166. doi: 10.1016/j.vetpar.2016.08.025
Pleasance, J., Wiedosari, E., Raadsma, H. W., Meeusen, E., & Piedrafita, D. (2011). Resistance to liver fluke infection in the natural sheep host is correlated with a type-1 cytokine response. Parasite Immunology, 33(9), 495-505. doi: 10.1111/j.1365-3024.2011.01305.x
Ravida, A., Aldridge, A. M., Driessen, N. N., Heus, F. A., Hokke, C. H., & O'Neill, S. M. (2016). Fasciola hepatica surface coat glycoproteins contain Mannosylated and phosphorylated N-glycans and exhibit immune modulatory properties independent of the mannose receptor. PLOS Neglected Tropical Diseases, 10(4), e0004601. doi: 10.1371/journal.pntd.0004601
Rojo-Vazquez, F. A., Meana, A., Valcarcel, F., & Martinez-Valladares, M. (2012). Update on trematode infections in sheep. Veterinary Parasitology, 189(1), 15-38. doi: 10.1016/j.vetpar.2012.03.029
Ruiz-Campillo, M. T., Hernandez, V. M., Escamilla, A., Stevenson, M., Perez, J., Martinez-Moreno, Donnelly, S., Dalton, J. P. & Cwiklinski, K. (2017). Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica. Scientific Reports, 7(2782), 1-14. doi: 10.1038/s41598-017-03094-0
Vukman, K. V., Adams, P. N., & O'Neill, S. M. (2013b). Fasciola hepatica tegumental coat antigen suppresses MAPK signalling in dendritic cells and up-regulates the expression of SOCS3. Parasite Immunology, 35(7-8), 234-238. doi: 10.1111/pim.12033
Vukman, K. V., Adams, P. N., Metz, M., Maurer, M., & O'Neill, S. M. (2013a). Fasciola hepatica tegumental coat impairs mast cells' ability to drive Th1 immune responses. The Journal of Immunology, 190(6), 2873-2879. doi: 10.4049/jimmunol.1203011
Zhang, W. Y., Moreau, E., Hope, J. C., Howard, C. J., Huang, W. Y., & Chauvin, A. (2005). Fasciola hepatica and Fasciola gigantica: comparison of cellular response to experimental infection in sheep. Experimental Parasitology, 111(3), 154-159. doi: 10.1016/j.exppara.2005.06.005
Beesley, N. J., Williams, D. J. L., Paterson, S., & Hodgkinson, J. (2017). Fasciola hepatica demonstrates high levels of genetic diversity, a lack of population structure and high gene flow: possible implications for drug resistance. International Journal for Parasitology, 47(1), 11-20. doi:10.1016/j.ijpara.2016.09.0 07
Boray, J. C. (1967). Studies on experimental infections with Fasciola hepatica, with particular reference to acute fascioliasis in sheep. Annals of Tropical Medicine & Parasitology, 61(4), 439-450. doi: 10.1080/0 0034983.1967.11686513
Chauvin, A., Bouvet, G., & Boulard, C. (1995). Humoral and cellular immune responses to Fasciola hepatica experimental primary and secondary infection in sheep. International Journal for Parasitology, 25(10), 1227-1241. doi: 10.1016/0020-7519(95)00039-5
Dalton, J. P., Robinson, M. W., Mulcahy, G., O'Neill, S. M., & Donnelly, S. (2013). Immunomodulatory molecules of Fasciola hepatica: candidates for both vaccine and immunotherapeutic development. Veterinary Parasitology, 195(3-4), 272-285. doi: 10.1016/j.vetpar.2013.04.
Dracz, R. M., & Lima, W. S. (2014). Autochthonous infection of buffaloes and cattle by Fasciola hepatica in Minas Gerais, Brazil. Revista Brasileira de Parasitologia Veterinária, 23(3), 413-416. doi: 10.1590/S1 984-29612014054
Dracz, R. M., Ribeiro, V. M. A., Pereira, C. A. J., & Lima, W. S. (2016). Ocorrência de Fasciola hepatica (Linnaeus, 1758) em capivara (Hydrochoerus hydrochaeris) (Linnaeus, 1766) em Minas Gerais, Brasil. Revista Brasileira de Parasitologia Veterinária, 25(3), 364-367. doi: 10.1590/S1984-29612016021
Flynn, R. J., & Mulcahy, G. (2008). The roles of IL-10 and TGF-beta in controlling IL-4 and IFN-gamma production during experimental Fasciola hepatica infection. International Journal for Parasitology, 38(14), 1673-1680. doi: 10.1016/j.ijpara.2008.05.008
Flynn, R. J., & Musah-Eroje, M. (2020). Evasion of Host Immunity during Fasciola hepatica Infection. In M. Cancela, & G. Maggioli (Eds.), Fasciola hepatica: Methods and Protocols, Methods in Molecular Biology. (Vol. 2137, pp. 107-115). Recovered from https://doi.org/10.1007/978-1-0716-0475-5_1,© Springer Science Business Media, LLC, part of Springer Nature 2020
Furst, T., Duthaler, U., Sripa, B., Utzinger, J., & Keiser, J. (2012). Trematode infections: liver and lung flukes. Infectious disease clinics, 26(2), 399-419. doi: 10.1016/j.idc.2012.03.008
Hacariz, O., Sayers, G., Flynn, R. J., Lejeune, A., & Mulcahy, G. (2009). IL-10 and TGF-beta1 are associated with variations in fluke burdens following experimental fasciolosis in sheep. Parasite Immunology, 31(10), 613-622. doi: 10.1111/j.1365-3024.2009.01135.x
Lima, W. dos S., Soares, L. R., Barcante, T. A., Guimaraes, M. P., & Barcante, J. M. (2009) Occurrence of Fasciola hepatica (Linnaeus, 1758) infection in Brazilian cattle of Minas Gerais, Brazil. Revista Brasileira de Parasitologia Veterinária, 18(2), 27-30. doi: 10.4322/rbpv.01802006
Mendes, E. A., Mendes, T. A. O., Santos, S. L., Menezes-Souza, D., Bartholomeu, D. C., Martins, I. V. F., Silva, L.M., Lima, W. S. (2013). Expression of IL-4, IL-10 and IFN-gamma in the liver tissue of cattle that are naturally infected with Fasciola hepatica. Veterinary Parasitology, 195(1-2), 177-182. doi: 10. 1016/j.vetpar.2013.03.035
Moreau, E., & Chauvin, A. (2010). Immunity against helminths: interactions with the host and the intercurrent infections. Journal of Biomedicine and Biotechnology, 2010 (428593), 1-10. doi: 10.1155/ 2010/428593
Moreau, E., Herve, S., Yu, Z. W., & Alain, C. (2002). Modulation of sheep lymphocyte responses by Fasciola hepatica excretory-secretory products. Veterinary Parasitology, 108(3), 207-215. doi: 10. 1016/S0304-4017(02)00196-6
Moxon, J. V., Flynn, R. J., Golden, O., Hamilton, J. V., Mulcahy, G., & Brophy, P. M. (2010). Immune responses directed at egg proteins during experimental infection with the liver fluke Fasciola hepatica. Parasite Immunology, 32(2), 111-124. doi: 10.1111/j.1365-3024.2009.01171.x
Pereira, C. A. J., Oliveira, L. L. S., Coaglio, A. L., Santos, F. S. O., Cezar, R. S. M., Mendes, T., Oliveira F. L. P., Consenza, G. Lima, W. S. (2016). Anti-helminthic activity of Momordica charantia L. against Fasciola hepatica eggs after twelve days of incubation in vitro. Veterinary Parasitology, 228(15), 160-166. doi: 10.1016/j.vetpar.2016.08.025
Pleasance, J., Wiedosari, E., Raadsma, H. W., Meeusen, E., & Piedrafita, D. (2011). Resistance to liver fluke infection in the natural sheep host is correlated with a type-1 cytokine response. Parasite Immunology, 33(9), 495-505. doi: 10.1111/j.1365-3024.2011.01305.x
Ravida, A., Aldridge, A. M., Driessen, N. N., Heus, F. A., Hokke, C. H., & O'Neill, S. M. (2016). Fasciola hepatica surface coat glycoproteins contain Mannosylated and phosphorylated N-glycans and exhibit immune modulatory properties independent of the mannose receptor. PLOS Neglected Tropical Diseases, 10(4), e0004601. doi: 10.1371/journal.pntd.0004601
Rojo-Vazquez, F. A., Meana, A., Valcarcel, F., & Martinez-Valladares, M. (2012). Update on trematode infections in sheep. Veterinary Parasitology, 189(1), 15-38. doi: 10.1016/j.vetpar.2012.03.029
Ruiz-Campillo, M. T., Hernandez, V. M., Escamilla, A., Stevenson, M., Perez, J., Martinez-Moreno, Donnelly, S., Dalton, J. P. & Cwiklinski, K. (2017). Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica. Scientific Reports, 7(2782), 1-14. doi: 10.1038/s41598-017-03094-0
Vukman, K. V., Adams, P. N., & O'Neill, S. M. (2013b). Fasciola hepatica tegumental coat antigen suppresses MAPK signalling in dendritic cells and up-regulates the expression of SOCS3. Parasite Immunology, 35(7-8), 234-238. doi: 10.1111/pim.12033
Vukman, K. V., Adams, P. N., Metz, M., Maurer, M., & O'Neill, S. M. (2013a). Fasciola hepatica tegumental coat impairs mast cells' ability to drive Th1 immune responses. The Journal of Immunology, 190(6), 2873-2879. doi: 10.4049/jimmunol.1203011
Zhang, W. Y., Moreau, E., Hope, J. C., Howard, C. J., Huang, W. Y., & Chauvin, A. (2005). Fasciola hepatica and Fasciola gigantica: comparison of cellular response to experimental infection in sheep. Experimental Parasitology, 111(3), 154-159. doi: 10.1016/j.exppara.2005.06.005
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